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adenylate kinase may lead to conversion of ADP into ATP and AMP and thereby confound interpretation in the ADP/ATP specificity experiments. Solving the conundrum of how some twin-specificity archaeal ligases could possibly employ ATP and NAD+ will rely on capturing crystal constructions of such enzymes with Every single nucleotide bound.

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). The NTase domain binds on the broken and intact DNA strands in the most important groove flanking the nick and also within the slight groove about the three′-OH aspect Ufabet Gambling Sites on the nick (supplemental Fig. S3). The OB domain binds throughout the minimal groove to the face of your duplex at the rear of the nick.

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